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Novus Biologicals
anti mpc1 Anti Mpc1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/anti mpc1/product/Novus Biologicals Average 93 stars, based on 1 article reviews
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Novus Biologicals
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OriGene
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Absolute Biotech Inc
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OriGene
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Image Search Results
Journal: American Journal of Translational Research
Article Title: Artemether regulates liver glycogen and lipid utilization through mitochondrial pyruvate oxidation in db/db mice
doi:
Figure Lengend Snippet: Effect of artemether on RER and the major proteins involved in pyruvate oxidation in liver. A, B. RER values for the dark and light phase in each group. n=4 per group. C. Immunoblotting bands of p-PDH, PDH, PDK1, PDK4, MPC1, and MPC2 in various groups. D-I. Bar graphs displaying the fold changes of p-PDH, PDH, PDK1, PDK4, MPC1, and MPC2 in different groups. n=4 per group. *P<0.05, **P<0.01 and ***P<0.001 vs. control group; #P<0.05, ##P<0.01 and ###P<0.001 vs. the db/db group. J. Representative immunohistochemical staining images of PDK1 from each group. Scale bar, 100 µm.
Article Snippet: After blocking, the membranes were incubated overnight at 4°C with the following primary antibodies: pyruvate dehydrogenase (PDH; CST#2784), p-PDH (Ser293) (CST#31866), PDK1 (Enzo Life Science; ADI-KAP-PK112-F), pyruvate dehydrogenase kinase 4 (PDK4; Proteintech; 12949-1-AP),
Techniques: Western Blot, Control, Immunohistochemical staining, Staining
Journal: Scientific Reports
Article Title: Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes
doi: 10.1038/s41598-020-59709-6
Figure Lengend Snippet: Effects of down regulating related genes in DOs or CCM on in vitro maturation of pig oocytes. Graph ( A ) shows percentages of MII oocytes after DOs were cultured for 48 h in the NCSU-23 medium containing 0, 10 or 15 mM pyruvate (Pyr) with 10, 25 or 50 µM 4-CIN (CIN) or with 0.01, 0.03 or 0.05 µM rotenone (Rot). Graph B shows percentages of MII oocytes after DOs were cultured for 48 h in the NCSU-23 medium containing 0, 5 or 10 mM lactate (Lac) with 10, 25 or 50 mM sodium oxamate (Oxm) or with 10, 20 or 50 µM 4-CIN. Graphs ( C–E ) show MII percentages of cocultured DOs (Left Y axis) and levels of MPC1, NDUFV1 and LDHB (Right Y axis) after transfection of CCM with negative control (NC) siRNA, MPC1 siRNA (MP1, 2 or 3), NDUFV1 siRNA (ND1, 2 or 3), or LDHB siRNA (LD1, 2 or 3), respectively. Graphs ( F,G ) show MII percentages of DOs after microinjection with NC, MP3, ND1 or LD2 siRNAs. While DOs and the CCM transfected/injected with MPC1 or NDUFV1 siRNAs were cultured in NCSU-23 containing 15 mM pyruvate, DOs and the CCM transfected/injected with LDHB siRNA were cultured in NCSU-23 containing 10 mM lactate. In oocyte maturation experiments, each treatment was repeated 4 times with each replicate containing about 20 oocytes. Graphs ( H,I ) show ATP concentrations (ng/ml) in medium conditioned with CCM in NCSU-23 medium containing 5.6 mM glucose and 15 mM pyruvate, respectively, after transfection with G6PD siRNA-3 (G6-3), GAPDH siRNA-3 (GA-3) or MPC1 siRNA-3 (MP3). Each treatment was repeated 3 times with each replicate containing medium recovered from one culture well on different experimental days. a–f: Values with a different letter above bars differ significantly (P < 0.05).
Article Snippet: The primary antibodies used included rabbit anti-G6PD monoclonal antibodies (1:1000, ab993, Abcam Co., Ltd, Beijing, China), mouse anti-GAPDH monoclonal antibodies (1:1000, CW0100A, CWBio Co., Ltd, Beijing, China), rabbit anti-LDHB polyclonal antibodies (1:1000, 14824-1-AP, Proteintech Co., Ltd, Wuhan, China),
Techniques: In Vitro, Cell Culture, Transfection, Negative Control, Injection
Journal: Scientific Reports
Article Title: Effects of glucose metabolism pathways on nuclear and cytoplasmic maturation of pig oocytes
doi: 10.1038/s41598-020-59709-6
Figure Lengend Snippet: Sequences of the sense strands of siRNAs targeting different genes.
Article Snippet: The primary antibodies used included rabbit anti-G6PD monoclonal antibodies (1:1000, ab993, Abcam Co., Ltd, Beijing, China), mouse anti-GAPDH monoclonal antibodies (1:1000, CW0100A, CWBio Co., Ltd, Beijing, China), rabbit anti-LDHB polyclonal antibodies (1:1000, 14824-1-AP, Proteintech Co., Ltd, Wuhan, China),
Techniques: